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6 March 2014 Microfluidic devices for cell culture and handling in organ-on-a-chip applications
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For many problems in system biology or pharmacology, in-vivo-like models of cell-cell interactions or organ functions are highly sought after. Conventional stationary cell culture in 2D plates quickly reaches its limitations with respect to an in-vivo like expression and function of individual cell types. Microfabrication technologies and microfluidics offer an attractive solution to these problems. The ability to generate flow as well as geometrical conditions for cell culture and manipulation close to the in-vivo situation allows for an improved design of experiments and the modeling of organ-like functionalities. Furthermore, reduced internal volumes lead to a reduction in reagent volumes necessary as well as an increased assay sensitivity. In this paper we present a range of microfluidic devices designed for the co-culturing of a variety of cells. The influence of substrate materials and surface chemistry on the cell morphology and viability for long-term cell culture has been investigated as well as strategies and medium supply for on-chip cell cultivation.
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Holger Becker, Ingo Schulz, Alexander Mosig, Tobias Jahn, and Claudia Gärtner "Microfluidic devices for cell culture and handling in organ-on-a-chip applications", Proc. SPIE 8976, Microfluidics, BioMEMS, and Medical Microsystems XII, 89760N (6 March 2014);

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