6 March 2014 Real-time PCR in microfluidic devices
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Abstract
A central method in a standard biochemical laboratory is represented by the polymerase chain reaction (PCR), therefore many attempts have been performed so far to implement this technique in lab-on-a-chip (LOC) devices. PCR is an ideal candidate for miniaturization because of a reduction of assay time and decreased costs for expensive bio-chemicals. In case of the “classical” PCR, detection is done by identification of DNA fragments electrophoretically separated in agarose gels. This method is meanwhile frequently replaced by the so-called Real-Time-PCR because here the exponential increase of amplificates can be observed directly by measurement of DNA interacting fluorescent dyes. Two main methods for on-chip PCRs are available: traditional “batch” PCR in chambers on a chip using thermal cycling, requiring about 30 minutes for a typical PCR protocol and continuous-flow PCR, where the liquid is guided over stationary temperature zones. In the latter case, the PCR protocol can be as fast as 5 minutes. In the presented work, a proof of concept is demonstrated for a real-time-detection of PCR products in microfluidic systems.
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Holger Becker, Nadine Hlawatsch, Richard Klemm, Christian Moche, Thomas Hansen-Hagge, Claudia Gärtner, "Real-time PCR in microfluidic devices", Proc. SPIE 8976, Microfluidics, BioMEMS, and Medical Microsystems XII, 89760Z (6 March 2014); doi: 10.1117/12.2037241; https://doi.org/10.1117/12.2037241
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