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7 March 2014 Adaptive optics for in vivo two-photon calcium imaging of neuronal networks
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Proceedings Volume 8978, MEMS Adaptive Optics VIII; 89780C (2014)
Event: SPIE MOEMS-MEMS, 2014, San Francisco, California, United States
The landscape of biomedical research in neuroscience has changed dramatically in recent years as a result of spectacular progress in dynamic microscopy. However, the optical accessibility of deep brain structures or deeper regions of the surgically exposed hippocampus (a few 100 microns typically) remains limited, due to volumic aberrations created by the sample inhomogeneities. Adaptive optics can correct for these aberrations. Our goal is to realize a novel adaptive optics module dedicated to in vivo two-photon calcium imaging of the hippocampus. The key issue in adaptive optics is the ability to perform an accurate and reliable wavefront sensing. In two- photon microscopy indirect methods are required. Two families of approaches have been proposed so far, the modal sensorless technique and a method based on pupil segmentation. We present here a formal comparison of these approaches, in particular as a function of the amount of aberrations.
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Serge Meimon, Jean-Marc Conan, Laurent M. Mugnier, Vincent Michau, Rosa Cossart, and Arnaud Malvache "Adaptive optics for in vivo two-photon calcium imaging of neuronal networks", Proc. SPIE 8978, MEMS Adaptive Optics VIII, 89780C (7 March 2014);

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