8 March 2014 A novel phase-sensitive SPR biosensor array based on prism phase modulator
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Phase modulators in surface plasmon resonance phase-differential imaging (SPR-PI) sensing systems reported so far are sensitive to temperature fluctuations or mechanical vibrations and thus their applications are limited. In this paper, we propose a novel prism phase modulator (PPM) to replace a traditional modulator. The PPM consists of a parallel prism, a rotation stage and a mirror. The PPM shows great stability in our experiment, and helps achieve high detection sensitivity in our SPR-PI system. Moreover, the cost of our PPM is much lower than that of a traditional modulator and is thus suitable for commercialization. A polydimethylsiloxane (PDMS) microfluidic chip is fabricated to control the flow velocity and realize parallel detection in our experiment. Measured result of glycerine solution shows that the resolution of our SPR biosensor array is about 9.11×10-7 refractive index unit (RIU). Real time monitoring of interaction between bovine IgG and anti-bovine IgG is also realized. The proposed PPM-based microfluidic SPR-PI biosensor array is promising for future practical applications.
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Gaoao Ye, Gaoao Ye, Wei Yang, Wei Yang, Li Jiang, Li Jiang, Jun Qian, Jun Qian, Sailing He, Sailing He, "A novel phase-sensitive SPR biosensor array based on prism phase modulator", Proc. SPIE 8989, Smart Photonic and Optoelectronic Integrated Circuits XVI, 89890Q (8 March 2014); doi: 10.1117/12.2047367; https://doi.org/10.1117/12.2047367

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