25 February 2014 Near-field waveguide trapping and tracking of particles using fluorescence imaging
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Proceedings Volume 8999, Complex Light and Optical Forces VIII; 899905 (2014) https://doi.org/10.1117/12.2039235
Event: SPIE OPTO, 2014, San Francisco, California, United States
Abstract
The evanescent field from an optical waveguide is used for near-field trapping and transporting of fluorescent microspheres. Out-of-focus fluorescence imaging is used to track the trapped particle in 3-D with nanometer precision (<100 nm). A prior calibration is done to determine the relationship between the z-coordinate and the radius of the outermost diffraction ring in the image of the sphere. This gives precise information about how much the particle moves up and down during propulsion along the waveguide. Results are presented for trapping and tracking a 1 μm fluorescent particle on a strip waveguide.
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Balpreet S. Ahluwalia, Balpreet S. Ahluwalia, Petter Brox, Petter Brox, Øystein Helle, Øystein Helle, Jean-Claude Tinguely, Jean-Claude Tinguely, Olav G. Hellesø, Olav G. Hellesø, } "Near-field waveguide trapping and tracking of particles using fluorescence imaging", Proc. SPIE 8999, Complex Light and Optical Forces VIII, 899905 (25 February 2014); doi: 10.1117/12.2039235; https://doi.org/10.1117/12.2039235
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