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30 January 2014 Optical tweezers-assisted measurements of elastic light scattering
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Abstract
Optical tweezers have been used in biophysical studies for over twenty years. Typical application areas are force measurements of subcellular structures and cell biomechanics. Optical tweezers can also be used to manipulate the orientation of objects. Moreover, using various beam shapes, optical tweezers allow measuring light scattering from single and multiple objects by keeping particles and cells in place during the measurement. At single cell level, light scattering yields important information about the object being studied, including its size, shape and refractive index. Also dependent scattering can be studied. In this paper, we review experimental work conducted in this area by our group and show new results relating to optical clearing phenomena at single microparticle level.
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M. Kinnunen, J. Tuorila, T. Haapalainen, A. Karmenyan, V. Tuchin, and R. Myllylä "Optical tweezers-assisted measurements of elastic light scattering", Proc. SPIE 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV, 90310A (30 January 2014); https://doi.org/10.1117/12.2051443
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