Applicability of diffusion approximation in analysis of diffuse reflectance spectra from healthy human skin

Peter Naglič; Luka Vidovič; Matija Milanič; Lise L. Randeberg; Boris Majaron

doi:10.1117/12.2044706

18 November 2013 Applicability of diffusion approximation in analysis of diffuse reflectance spectra from healthy human skin

Peter Naglič, Luka Vidovič, Matija Milanič, Lise L. Randeberg, Boris Majaron

Author Affiliations +

Proceedings Volume 9032, Biophotonics—Riga 2013; 90320N (2013) https://doi.org/10.1117/12.2044706
Event: 1st International Conference "Biophotonics Riga 2013", 2013, Riga, Latvia

Abstract

Measurement of diffuse reflectance spectra (DRS) is a common experimental approach for non-invasive determination of tissue optical properties, as well as objective monitoring of various tissue malformations. Propagation of light in scattering media is often treated in diffusion approximation (DA). The major advantage of this approach is that it leads to enclosed analytical solutions for tissues with layered structure, which includes human skin. Despite the fact that DA solutions were shown to be inaccurate near tissue boundaries, the practicality of this approach makes it quite popular, especially when attempting extraction of specific chromophore concentrations from measured DRS. In this study we analyze the discrepancies between DRS spectra as obtained by using the DA solutions for three-layer skin model and more accurate predictions from Monte Carlo (MC) modeling. Next, we analyze the artifacts which result from the above discrepancies when extracting the parameters of skin structure and composition by fitting the DA solutions to the MC spectra. The reliability and usefulness of such a fit is then tested also on measurements of seasonal changes in otherwise healthy human skin.

Citation Download Citation

Peter Naglič, Luka Vidovič, Matija Milanič, Lise L. Randeberg, and Boris Majaron "Applicability of diffusion approximation in analysis of diffuse reflectance spectra from healthy human skin", Proc. SPIE 9032, Biophotonics—Riga 2013, 90320N (18 November 2013); https://doi.org/10.1117/12.2044706

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available