Elastic light single-scattering spectroscopy for detection of dysplastic tissues

Murat Canpolat; Tuba Denkçeken; Ayşe Akman; Erkan Alpsoy; Recai Tuncer; Mahmut Akyüz; Mehmet Baykara; Selçuk Yücel; Ibrahim Başsorgun; M. Akif Çiftçioğlu; Güzide Ayşe Gökhan; Elifİnanç Gürer; Elif Peştereli; Şeyda Karaveli

doi:10.1117/12.2044681

18 November 2013 Elastic light single-scattering spectroscopy for detection of dysplastic tissues

Murat Canpolat, Tuba Denkçeken, Ayşe Akman, Erkan Alpsoy, Recai Tuncer, Mahmut Akyüz, Mehmet Baykara, Selçuk Yücel, Ibrahim Başsorgun, M. Akif Çiftçioğlu, Güzide Ayşe Gökhan, Elifİnanç Gürer, Elif Peştereli, Şeyda Karaveli

Author Affiliations +

Proceedings Volume 9032, Biophotonics—Riga 2013; 90320S (2013) https://doi.org/10.1117/12.2044681
Event: 1st International Conference "Biophotonics Riga 2013", 2013, Riga, Latvia

Abstract

Elastic light single-scattering spectroscopy (ELSSS) system has been developed and tested in diagnosis of cancerous tissues of different organs. ELSSS system consists of a miniature visible light spectrometer, a single fiber optical probe, a halogen tungsten light source and a laptop. Measurements were performed on excised brain, skin, cervix and prostate tumor specimens and surrounding normal tissues. Single fiber optical probe with a core diameter of 100 μm was used to deliver white light to and from tissue. Single optical fiber probe mostly detects singly scattered light from tissue rather than diffused light. Therefore, measured spectra are sensitive to size of scatters in tissue such as cells, nuclei, mitochondria and other organelles of cells. Usually, nuclei of tumor cells are larger than nuclei of normal cells. Therefore, spectrum of singly scattered light of tumor tissue is different than normal tissue. The spectral slopes were shown to be positive for normal brain, skin and prostate and cervix tissues and negative for the tumors of the same tissues. Signs of the spectral slopes were used as a discrimination parameter to differentiate tumor from normal tissues for the three organ tissues. Sensitivity and specificity of the system in differentiation between tumors from normal tissues were 93% and %100 for brain, 87% and 85% for skin, 93.7% and 46.1% for cervix and 98% and 100% for prostate.

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Murat Canpolat, Tuba Denkçeken, Ayşe Akman, Erkan Alpsoy, Recai Tuncer, Mahmut Akyüz, Mehmet Baykara, Selçuk Yücel, Ibrahim Başsorgun, M. Akif Çiftçioğlu, Güzide Ayşe Gökhan, Elifİnanç Gürer, Elif Peştereli, and Şeyda Karaveli "Elastic light single-scattering spectroscopy for detection of dysplastic tissues", Proc. SPIE 9032, Biophotonics—Riga 2013, 90320S (18 November 2013); https://doi.org/10.1117/12.2044681

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