16 April 2014 FITC-tagged macromolecule-based alginate microspheres for urea sensoring
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Abstract
Urea is an important biomarker for identification of kidney diseases. Early urea detection using a specific and sensitive technique can significantly reduce the mortality of patients. The research aims at developing fluorescence-based FITCmediated pH and urea measurement. A system containing FITC-dextran in alginate microspheres was developed using air-driven atomization. pH/Urea biosensor was characterized using optical microscopy, SEM, and CLSM. Urea biosensing studies were performed by exposing different standard solutions of pH and urea standard solutions using fluorescence spectroscopy (λex=488 nm and λem=520 nm). FITC-dextran was entrapped using an encapsulation unit and alginate microspheres were formed. The microspheres were found to be uniform and spherical in nature with sizes (50±10μ). FITC-dextran was found to be uniformly distributed in the alginate microspheres as per the CLSM scans. Urea biosensing studies indicate that a linear correlation was observed with increasing urea concentrations. The said microspheres can be used to detect changes in pH from 4-8 units owing to its linear response in this range. FITC dextran loaded alginate microspheres showed an improved range of detection upto 7 mM in comparison to 1.5 mM when in solution phase in a study with urea concentrations from 0-50 mM. The pH and urea detection was accurate to an extent of interday variation of 5%. FITC-dextran loaded alginate microspheres show a great potential for usage as a pH and urea biosensor for early detection of kidney diseases.
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Abhijeet Joshi, Abhijeet Joshi, Rashmi Chaudhari, Rashmi Chaudhari, Rohit Srivastava, Rohit Srivastava, } "FITC-tagged macromolecule-based alginate microspheres for urea sensoring", Proc. SPIE 9060, Nanosensors, Biosensors, and Info-Tech Sensors and Systems 2014, 90600P (16 April 2014); doi: 10.1117/12.2044898; https://doi.org/10.1117/12.2044898
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