A Global Instrumentation Approach To Resolution Of Ground-State And Excited-State Processes In Time-Resolved Fluorescence Spectroscopy

J.B.Alexander Ross; William R Laws; Herman R Wyssbrod

doi:10.1117/12.945371

24 June 1988 A Global Instrumentation Approach To Resolution Of Ground-State And Excited-State Processes In Time-Resolved Fluorescence Spectroscopy

J.B.Alexander Ross, William R Laws, Herman R Wyssbrod

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945371
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

The application of global analysis to time-dependent fluorescence data provides a power-ful technique for investigating complex, heterogeneous systems by defining common parameters to elements of a data set where the data has been obtained as a function of an independent variable. Another, related approach is to incorporate information obtained from other physical techniques into the analysis of time-dependent fluorescence data. The information from the independent method is used to define a linkage among the parameters within one element of the time-dependent fluorescence data set; the linkage establishes certain parameters as dependent variables. We call this a linked-function analysis, which can be used in the analysis of a single data set element or globally in the analysis of multiple data set elemets. This approach has been applied to time-domain fluorescence spectroscopy combi n ned with 1H-NMR spectroscopy to resolve ground-state and excited-state processes in the fluorescence decay of aromatic amino residues in peptides.

Citation Download Citation

J.B.Alexander Ross, William R Laws, and Herman R Wyssbrod "A Global Instrumentation Approach To Resolution Of Ground-State And Excited-State Processes In Time-Resolved Fluorescence Spectroscopy", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945371

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