Fluorescence Depolarization Studies Of Molecular Dynamics Using Pulsed Synchrotron Radiation: Applications To Lipid Vesicles And Oriented Multibilayers

Herman van Langen; Gijs van Ginkel; Yehudi K Levine

doi:10.1117/12.945417

24 June 1988 Fluorescence Depolarization Studies Of Molecular Dynamics Using Pulsed Synchrotron Radiation: Applications To Lipid Vesicles And Oriented Multibilayers

Herman van Langen, Gijs van Ginkel, Yehudi K Levine

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945417
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Analysis of time-resolved fluorescence anisotropy measurements on TMA-DPH molecules in POPC vesicles in terms of the rotational diffusion model reveals two distinct X²r minima which are statistically equivalent. The ambiguity in the interpretation is resolved by carrying out angle-resolved fluorescence depolarization experiments on planar POPC multibilayers in the time domain. It is shown that the order parameters of the probe molecules are higher in the multibilayers than in the vesicles. Furthermore the reorientational motion of the probes is considerably slower in the multibilayer sample. These differences are ascribed to the effects of the curvature and hydration of the bilayers in the vesicle systems.

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Herman van Langen, Gijs van Ginkel, and Yehudi K Levine "Fluorescence Depolarization Studies Of Molecular Dynamics Using Pulsed Synchrotron Radiation: Applications To Lipid Vesicles And Oriented Multibilayers", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945417

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