Gigahertz Frequency-Domain Fluorometry: Applications To Picosecond Processes And Future Developments

Joseph R Lakowicz; Gabor Laczko; Ignacy Gryczynski; Henryk Cherek; Wieslaw Wiczk

doi:10.1117/12.945364

24 June 1988 Gigahertz Frequency-Domain Fluorometry: Applications To Picosecond Processes And Future Developments

Joseph R Lakowicz, Gabor Laczko, Ignacy Gryczynski, Henryk Cherek, Wieslaw Wiczk

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945364
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

We describe our frequency-domain fluorometer which allows phase angle and demodulation measurements from 8 MHz to 2 GHz. This instrument using the harmonic content of a 7.59 MHz train of 5 ps pulses as the modulated excitation source. The detector is a microchannel plate PMT (R1564U), whose bandwidth extends to 2 GHz. Using this instrument we examined rapid rotational motions of indole in low viscosity solvents. To date, the shortest correlation time we have determined was 7 ps, for indole in methanol at 80°C. For indole in cyclohexane at 20°C we were able to resolve two rotational correlation times of 17 and 73 ps. It now appears that the frequency range can be extended to 8 GHz, so still faster processes should be observable.

Citation Download Citation

Joseph R Lakowicz, Gabor Laczko, Ignacy Gryczynski, Henryk Cherek, and Wieslaw Wiczk "Gigahertz Frequency-Domain Fluorometry: Applications To Picosecond Processes And Future Developments", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945364

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