28 May 2014 FRET based characterization of DNA-based assemblies
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The “spectroscopic ruler” based on fluorescence resonance energy transfer (FRET) is explored as a method for detailed structural characterization of DNA nanostructures in solution. The approach is most directly useful for assessing the positional relationships among chromophores organized by the DNA, but it can also be used to characterize the geometry and kinematics of the DNA scaffold itself. By accumulating data for the distances separating various donor-acceptor pairs, and correlating them with the expected distances, one can quantify the shape and deformability of the structure. A 8x16nm “mini-origami” rectangle is used as the model test structure and the dye-pairs are chosen to investigate anisotropy in the origami’s mechanical properties. Not unexpectedly, our analysis finds a strong anisotropy in the stiffness, with the measured spacing across the origami weave deviating much more from expectation than the spacing aligned along the weave pattern.
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Susan Buckhout-White, Susan Buckhout-White, Rochester Gray, Rochester Gray, Mario Ancona, Mario Ancona, Ellen R. Goldman, Ellen R. Goldman, Igor L. Medintz, Igor L. Medintz, "FRET based characterization of DNA-based assemblies", Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 91070Y (28 May 2014); doi: 10.1117/12.2050923; https://doi.org/10.1117/12.2050923

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