Monitoring enzyme kinetic behavior of enzyme-quantum dot bioconjugates

Jonathan C. Claussen; Scott A. Walper; Kimihiro Susumu; Mario G. Ancona; Igor L. Medintz

doi:10.1117/12.2050791

22 May 2014 Monitoring enzyme kinetic behavior of enzyme-quantum dot bioconjugates

Jonathan C. Claussen, Scott A. Walper, Kimihiro Susumu, Mario G. Ancona, Igor L. Medintz

Proceedings Volume 9107, Smart Biomedical and Physiological Sensor Technology XI; 910710 (2014) https://doi.org/10.1117/12.2050791
Event: SPIE Sensing Technology + Applications, 2014, Baltimore, MD, United States

Abstract

Luminescent semiconductor nanocrystals or quantum dots (QDs) hold tremendous promise for in vivo biosensing, cellular imaging, theranostics, and smart molecular sensing probes due to their small size and favorable photonic properties such as resistance to photobleaching, size-tunable PL, and large effective Stokes shifts. Herein, we demonstrate how QD-based bioconjugates can be used to enhance enzyme kinetics. Enzyme-substrate kinetics are analyzed for solutions containing both alkaline phosphatase enzymes and QDs with enzyme-to- QD molar ratios of 2, 12, and 24 as well as for a solution containing the same concentration of enzymes but without QDs. The enzyme kinetic paramters V_max, K_M, and K_cat/K_M are extracted from the enzyme progress curves via the Lineweaver-Burk plot. Results demonstrate an approximate increase in enzyme efficiency of 5 - 8% for enzymes immobilized on the QD versus free in solution without QD immobilization.

Citation Download Citation

Jonathan C. Claussen, Scott A. Walper, Kimihiro Susumu, Mario G. Ancona, and Igor L. Medintz "Monitoring enzyme kinetic behavior of enzyme-quantum dot bioconjugates", Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 910710 (22 May 2014); https://doi.org/10.1117/12.2050791

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