5 June 2014 Raman spectroscopic analysis of whole blood acetylcholinesterase
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Abstract
Raman spectra were taken from whole sheep’s blood with varying levels of acetylcholinesterase (AChE) inhibition using 229 and 532 nm laser excitation wavelengths. AChE levels were inhibited using the organophosphates malathion, paraoxon-ethyl, and octamethyldiphosphoramide and confirmed using the Ellman method. This AChE activity level was investigated with the Raman spectra and analyzed using a partial least squares calibration and cross validation to determine if the AChE activity could be predicted from the Raman spectrum. Correlation scores of 0.78 and 0.26 between the measured and predicted AChE activity were observed using 229 and 532 nm excitation, respectively. A estimate limit of detection was found to be approximately 0.01 ΔA/min.
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Phillip G. Wilcox, Jin U. Kang, "Raman spectroscopic analysis of whole blood acetylcholinesterase", Proc. SPIE 9112, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring IV, 91120V (5 June 2014); doi: 10.1117/12.2052701; https://doi.org/10.1117/12.2052701
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