Single gold nanoparticles to enhance the detection of single fluorescent molecules at micromolar concentration using fluorescence correlation spectroscopy

Deep Punj; Hervé Rigneault; Jérôme Wenger

doi:10.1117/12.2049910

2 May 2014 Single gold nanoparticles to enhance the detection of single fluorescent molecules at micromolar concentration using fluorescence correlation spectroscopy

Deep Punj, Hervé Rigneault, Jérôme Wenger

Author Affiliations +

Proceedings Volume 9126, Nanophotonics V; 91261N (2014) https://doi.org/10.1117/12.2049910
Event: SPIE Photonics Europe, 2014, Brussels, Belgium

Abstract

Single nanoparticles made of noble metals are strongly appealing to develop practical applications to detect fluorescent molecules in solution. Here, we detail the use of a single gold nanoparticle of 100 nm diameter to enhance the detection of single Alex Fluor 647 fluorescent molecules at high concentrations of several micromolar. We discuss the implementation of fluorescence correlation spectroscopy, and provide a new method to reliably extract the enhanced fluorescence signal stemming from the nanoparticle near-field from the background generated in the confocal volume. The applicability of our method is checked by reporting the invariance of the single molecule results as function of the molecular concentration, and the experimental data is found in good agreement with numerical simulations.

Citation Download Citation

Deep Punj, Hervé Rigneault, and Jérôme Wenger "Single gold nanoparticles to enhance the detection of single fluorescent molecules at micromolar concentration using fluorescence correlation spectroscopy", Proc. SPIE 9126, Nanophotonics V, 91261N (2 May 2014); https://doi.org/10.1117/12.2049910

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