2 May 2014 3D photonic crystal-based biosensor functionalized with quantum dot-based aptamer for thrombine detection
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Abstract
In this paper, we propose a new technique for protein detection by using the enhancement of intensity in quantum dots (Qdot) whose emission is guided by 3D photonic crystal (PC) structures. For easy to use, we design the emitted light from the sensor can be recovered, when the chemical antibody (aptamer) conjugated with guard DNA (g-DNA) labeled with a quencher (Black FQ) hybridizes with the target proteins. In detail, we synthesis a Qdot-aptamer complex and then immobilize these complex on the PC surfaces. Next, we perform the hybridization of the Qdot-aptamer complex with g-DNA labeled with the quencher. It induces the quenching effect of fluoresce intensity in the Qdot-aptamer. In presence of target protein (thrombin), the Qdot-aptamer complex prefers to form the thrombin-aptamer complex: this results in the release of Black FQ-g-DNA and the quenched light intensity recovers into the original high intensity with Qdot. The intensity recovery varies quantitatively according to the level of the target protein concentration. This proposed sensor shows much higher detection sensitivity than the general fluorescent detection mechanism, which is functionalized on the flat surfaces because of the light guiding effect from 3D photonic crystal structures.
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Chae Young Lim, Chae Young Lim, Eunpyo Choi, Eunpyo Choi, Youngkyu Park, Youngkyu Park, Jungyul Park, Jungyul Park, } "3D photonic crystal-based biosensor functionalized with quantum dot-based aptamer for thrombine detection", Proc. SPIE 9127, Photonic Crystal Materials and Devices XI, 91270W (2 May 2014); doi: 10.1117/12.2054149; https://doi.org/10.1117/12.2054149
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