15 May 2014 Collagen bioengineered systems: in situ advanced optical spatiotemporal analysis
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The architecture of collagen is important in maintenance and regeneration of higher vertebrates’ tissues. We had been studying the changes to this architecture with in situ multi-photon optical microscopy that combines nonlinear optical phenomena of second harmonic generation (SHG) and two-photon fluorescence (TPF) signals from collagen hydrogels prepared from different collagen solid content, polymerized at different temperatures, with different ions as well as modified with reducing sugars. We incubated 2 g/l collagen hydrogels with 0.1 M fructose at 37 °C and after about 20 days observed a significant induction of in situ fluorescence. The twophoton fluorescence emission was centered at about 460 nm for 730 nm excitation wavelength and shifted to 480 nm when we changed the excitation wavelength to 790 nm. The one-photon fluorescence emission was centered at about 416 nm when excitation was 330 nm. It red shifted and split into two peaks centered at about 430 nm and 460 nm for 370 nm excitation; 460 nm peak became predominant for 385 nm excitation and further shifted to 470 nm for 390 nm excitation. SHG and TPF imaging showed restructuring of hydrogels upon this modification. We will discuss these findings within the context of our ongoing dermal wound repair research.
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Yu Jer Hwang, Yu Jer Hwang, Xuye Lang, Xuye Lang, Joseph Granelli, Joseph Granelli, Cassandra C. Turgman, Cassandra C. Turgman, Jackie Gigante, Jackie Gigante, Julia G. Lyubovitsky, Julia G. Lyubovitsky, "Collagen bioengineered systems: in situ advanced optical spatiotemporal analysis", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291H (15 May 2014); doi: 10.1117/12.2052661; https://doi.org/10.1117/12.2052661

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