8 May 2014 Optical tweezers experiments for fibroblast cell growth stimulation
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Abstract
Optical tweezers constitute an increasingly used tool for the study of biomechanical properties of cells. Here we report experiments for the projection induction of NIH3T3 fibroblast cells, using a single-trap optical tweezers. The system is based on a 1064-nm, 50mW infrared gaussian laser beam, a 100x microscope objective with 1.25 numerical aperture and a temperature-controlled warming plate to maintain cell viability. Eighteen cells were exposed to the focussed laser beam in different cell zones and another 18 cells were observed without laser stimulation as a control population. The results show that the probability of lamelipodia growth increases on exposed cells by a factor 1.5.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Remy Avila, Remy Avila, Norma Medina-Villalobos, Norma Medina-Villalobos, Elisa Tamariz, Elisa Tamariz, Roger Chiu, Roger Chiu, Luz María Lopez-Marín, Luz María Lopez-Marín, Angélica Acosta, Angélica Acosta, Victor Castaño, Victor Castaño, "Optical tweezers experiments for fibroblast cell growth stimulation", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291U (8 May 2014); doi: 10.1117/12.2064939; https://doi.org/10.1117/12.2064939
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