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8 May 2014 Nonlinear and nonmonotonic nature of the intensity of fluorescence emission on a surface of turbid fluorescing biotissues
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Many researchers during past 20 years have used the laser fluorescence spectroscopy (LFS) for in vivo tissue diagnosis. But in the up-to-date medical in vivo LFS there is a problem of quantification of the fluorophores concentrations in optically-turbid biotissues basing on measurements of the laser induced fluorescence on a surface of the tissues. The purpose of our work is both experimental and theoretical study of the character of dependences of measured fluorescence intensities on tissues’ optical properties and on fluorophores concentrations in tissues. In the experimental part of our study the measurements of the superficial fluorescence on phantoms at different known concentration of fluorophores in them were carried out. As a result experimental dependences of the registered intensities of the laser induced fluorescence emission on fluorophores concentration were plotted. In the theoretical part of our study the analytical solution for a fluorescence emission by Kokhanovsky’s method based on the well-known two-flux Kubelka-Munk approach (KMA) was used. Besides, in the study the Kokhanovsky’s method was modified by its association with our improved KMA, allowing us to receive exact solutions for boundary intensities collected by an optical probe. As a result a set of theoretical curves describing the influence of fluorophore concentration in tissues on the registered intensities was obtained as well. Both experimental and theoretical results show a good qualitative agreement between each other. Also these results show that the dependence of the fluorescence intensity from tissues’ optical properties and from the concentration of fluorophores can be both nonlinear and non-monotonic.
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Dmitry Rogatkin, Ludmila Lapaeva, and Irina Guseva "Nonlinear and nonmonotonic nature of the intensity of fluorescence emission on a surface of turbid fluorescing biotissues", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91292H (8 May 2014);

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