8 May 2014 Raman spectroscopy and SERS analysis of ovarian tumour derived exosomes (TEXs): a preliminary study
Author Affiliations +
Abstract
Here we report a preliminary study based on the application of Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) to investigate the compositional differences between exosomes derived from ovarian carcinoma cells (cell line A2780) grown in normoxia (normal O2 conditions) and hypoxia (1% O2 conditions). Exosomes are integral to cell signalling, and are of interest in the study of how cells communicate within their environment. We are particularly interested in identifying whether hypoxia induced senescent cells can communi- cate via exosomes with neighbouring tumour cells, thereby causing them to become senescent and therefore radio and chemo resistant. With this goal in mind, we performed a preliminary study on the application of Raman spectroscopy and SERS to analyse the biomolecular fingerprint of both groups of exosomes and to investigate whether there exists a different biomolecular composition associated with exosomes derived from hypoxic cells in comparison to those from normoxic cells. We also applied multivariate statistical techniques for the classification of both groups of exosomes.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Laura T. Kerr, Laura T. Kerr, Luke Gubbins, Luke Gubbins, Karolina Weiner Gorzel, Karolina Weiner Gorzel, Shiva Sharma, Shiva Sharma, Malcolm Kell, Malcolm Kell, Amanda McCann, Amanda McCann, Bryan M. Hennelly, Bryan M. Hennelly, } "Raman spectroscopy and SERS analysis of ovarian tumour derived exosomes (TEXs): a preliminary study", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91292Q (8 May 2014); doi: 10.1117/12.2051759; https://doi.org/10.1117/12.2051759
PROCEEDINGS
9 PAGES


SHARE
Back to Top