8 May 2014 Two-step Raman spectroscopy method for tumor diagnosis
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Abstract
Two-step Raman spectroscopy phase method was proposed for differential diagnosis of malignant tumor in skin and lung tissue. It includes detection of malignant tumor in healthy tissue on first step with identification of concrete cancer type on the second step. Proposed phase method analyze spectral intensity alteration in 1300-1340 and 1640-1680 cm-1 Raman bands in relation to the intensity of the 1450 cm-1 band on first step, and relative differences between RS intensities for tumor area and healthy skin closely adjacent to the lesion on the second step. It was tested more than 40 ex vivo samples of lung tissue and more than 50 in vivo skin tumors. Linear Discriminant Analysis, Quadratic Discriminant Analysis and Support Vector Machine were used for tumors type classification on phase planes. It is shown that two-step phase method allows to reach 88.9% sensitivity and 87.8% specificity for malignant melanoma diagnosis (skin cancer); 100% sensitivity and 81.5% specificity for adenocarcinoma diagnosis (lung cancer); 90.9% sensitivity and 77.8% specificity for squamous cell carcinoma diagnosis (lung cancer).
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V. P. Zakharov, V. P. Zakharov, I. A. Bratchenko, I. A. Bratchenko, S. V. Kozlov, S. V. Kozlov, A. A. Moryatov, A. A. Moryatov, O. O. Myakinin, O. O. Myakinin, D. N. Artemyev, D. N. Artemyev, } "Two-step Raman spectroscopy method for tumor diagnosis", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91293V (8 May 2014); doi: 10.1117/12.2052121; https://doi.org/10.1117/12.2052121
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