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8 May 2014 Strategy for high recovery of fluorescence from quencher assembled quantum-dot donor
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Abstract
We fabricated 1.4 nm nanogold and molecular dark quencher assembled quantum dot for estimating their performances in a target specific conformal changing molecular event. For the assembling, we immobilized each acceptor linked molecular beacons using interaction between biotin at molecular beacon and streptavidins on quantum dot. Through optical analysis of the purified hybrids of the acceptors and quantum dots, we could estimate numbers of the assembled acceptors per quantum dot and their efficiency of energy transfer depending on conformal changes of molecular beacons. We obtained maximum 95 % and 78% of energy transfer efficiency with 17 metallic nanocrystals and 41 black hole quencher 2, the molecular dark quencher per single quantum dote, respectively. Molecular beacons form linear helix from a hair-pin structure by hybridizing with complementary DNA. In the presence of target DNA, energy transfer efficiency of the organic quencher was 22 % while only 2 % decreased efficiency was obtained with the nanogold, indicating higher fluorescence recovery with the ordinary organic quencher. Considering the relatively low assembled number and the large size, a steric hindrance might be attributed to the low fluorescence recovery. Since the energy transfer efficiency obtained with the nanogold at a fixed distance is high enough, it would be still effective to apply nanogold a system, where nanogold is removed permanently from quantum dots.
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Joong Hyun Kim and Mihrimah Ozkan "Strategy for high recovery of fluorescence from quencher assembled quantum-dot donor", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91293Y (8 May 2014); https://doi.org/10.1117/12.2053946
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