SIMBIOSYS is a highly integrated instrument suite that will be mounted on-board BepiColombo, which is the fifth cornerstone mission of the European Space Agency dedicated to the exploration of the planet Mercury and it is expected to be launched in 2016. The SIMBIOSYS instrument consists of three channels: the STereo imaging Channel (STC), with broad spectral bands in the 400–950 nm range and medium spatial resolution (up to 50 m/px); the High Resolution Imaging Channel (HRIC), with broad spectral bands in the 400–900 nm range and high spatial resolution (up to 5 m/px), and the Visible and near- Infrared Hyperspectral Imaging channel (VIHI), with high spectral resolution (up to 6 nm) in the 400–2000 nm range and spatial resolution up to 100 m/px. The on-ground calibration system has to cover the full spectral range of the instrument, i.e. from 400 to 2000 nm, and the emitted radiance has to vary over a range of four decades to account for both simulations of Mercury surface acquisition and star field observations. The methods and the results of the measurements done to calibrate the integrating sphere needed for the on-ground radiometric testing of the SIMBIOSYS instrument will be given and discussed. Temporal stability, both on short and long periods, spatial and spectral uniformity, and the emitted radiance for different lamp configurations and different shutter apertures have been measured. The results of the data analysis confirm that the performance of the integrating sphere is well suited for the radiometric calibration of all the three different channels of the SIMBIOSYS instrument.