ASTRI SST-2M is the end-to-end prototype telescope under development by the Italian National Institute of Astrophysics, INAF, proposed for the investigation of the highest-energy gamma-ray band in the framework of the Cherenkov Telescope Array, CTA. The ASTRI SST-2M prototype will be installed in Italy at the INAF station located at Serra La Nave on Mount Etna during Fall 2014. The calibration and scientific validation phase will start soon after. The calibration of a Cherenkov telescope includes several items and tools. The ASTRI SST- 2M camera is equipped with an internal fiber illumination system that allows to perform the relative calibration through monitoring of gain and efficiency variations of each pixel. The absolute calibration of the overall system, including optics, will take advantage from auxiliary instrumentation, namely UVscope and UVSiPM, two small-aperture multi-pixels photon detectors NIST calibrated in lab. During commissioning phase, to measure the main features of ASTRI SST-2M, as its overall spectral response, the main telescope and the auxiliary UVscope-UVSiPM will be illuminated simultaneously by a spatially uniform flux generated by a ground-based light source, named Illuminator, placed at a distance of few hundreds meters. Periodically, during clear nights, the flux profiles of a reference star tracked simultaneously by ASTRI SST-2M and UVscope-UVSiPM will allow to evaluate the total atmospheric attenuation and the absolute calibration constant of the ASTRI SST-2M prototype. In this contribution we describe the auxiliary UVscope-UVSiPM and Illuminator sub-system together with an overview of the end-to-end calibration procedure foreseen for the ASTRI SST-2M telescope prototype.