Rapid multiplexed molecular phenotyping of ex vivo and in vivo tissues with targeted SERS NPs

Yu Wang; Altaz Khan; Madhura Som; Steven Y. Leigh; Danni Wang; Ye Chen; Patrick McVeigh; Brian C. Wilson; Jonathan T. C. Liu

doi:10.1117/12.2057716

19 May 2014 Rapid multiplexed molecular phenotyping of ex vivo and in vivo tissues with targeted SERS NPs

Yu Wang, Altaz Khan, Madhura Som, Steven Y. Leigh, Danni Wang, Ye Chen, Patrick McVeigh, Brian C. Wilson, Jonathan T. C. Liu

Author Affiliations +

Proceedings Volume 9155, Translational Biophotonics; 91550Z (2014) https://doi.org/10.1117/12.2057716
Event: SPIE Translational Biophotonics, 2014, Houston, Texas, United States

Abstract

We are developing a miniature fiber-optic spectral-detection device and topical-staining protocol to rapidly detect multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) targeted to cell-surface biomarkers in fresh tissues. Ex vivo and in vivo experiments were performed to optimize our strategy for the rapid detection of multiple cell-surface biomarkers following a brief (5 min) topical application of SERS NPs on tissues. The simultaneous detection and ratiometric quantification of targeted and nontargeted NPs allows for an unambiguous assessment of molecular expression that is insensitive to nonspecific variations in NP concentrations, potentially enabling point-of-care surgical guidance or early disease detection.

Citation Download Citation

Yu Wang, Yu Wang, Altaz Khan, Altaz Khan, Madhura Som, Madhura Som, Steven Y. Leigh, Steven Y. Leigh, Danni Wang, Danni Wang, Ye Chen, Ye Chen, Patrick McVeigh, Patrick McVeigh, Brian C. Wilson, Brian C. Wilson, Jonathan T. C. Liu, Jonathan T. C. Liu, "Rapid multiplexed molecular phenotyping of ex vivo and in vivo tissues with targeted SERS NPs", Proc. SPIE 9155, Translational Biophotonics, 91550Z (19 May 2014); doi: 10.1117/12.2057716; https://doi.org/10.1117/12.2057716

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years