Modulated alignment dual-axis (MAD) confocal microscopy for deep optical sectioning in tissues

Steven Y. Leigh; Ye Chen; Jonathan T. C. Liu

doi:10.1117/12.2057734

19 May 2014 Modulated alignment dual-axis (MAD) confocal microscopy for deep optical sectioning in tissues

Steven Y. Leigh, Ye Chen, Jonathan T. C. Liu

Author Affiliations +

Proceedings Volume 9155, Translational Biophotonics; 915513 (2014) https://doi.org/10.1117/12.2057734
Event: SPIE Translational Biophotonics, 2014, Houston, Texas, United States

Abstract

A strategy is presented to enable optical-sectioning microscopy with improved contrast and imaging depth using low-power (0.5 mW) diode laser illumination. While the DAC architecture’s intersecting illumination and collection beams significantly improves the spatial-filtering and opticalsectioning performance of confocal microscopy, we propose that modulating the spatial alignment of the dual-axis beams at a frequency f, such the focal volume signal of the microscope is modulated at 2f, further provides nearly an order-of-magnitude improvement in optical-sectioning contrast. Lock-in detection is used to remove the unmodulated background light, thereby enhancing our ability to image deeply within highly scattering tissues.

Citation Download Citation

Steven Y. Leigh, Steven Y. Leigh, Ye Chen, Ye Chen, Jonathan T. C. Liu, Jonathan T. C. Liu, "Modulated alignment dual-axis (MAD) confocal microscopy for deep optical sectioning in tissues", Proc. SPIE 9155, Translational Biophotonics, 915513 (19 May 2014); doi: 10.1117/12.2057734; https://doi.org/10.1117/12.2057734

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