2 June 2014 Real-time monitoring of intracellular signal transduction in PC12 cells by non-adiabatic tapered optical fiber biosensor
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Proceedings Volume 9157, 23rd International Conference on Optical Fibre Sensors; 91574I (2014) https://doi.org/10.1117/12.2058939
Event: OFS2014 23rd International Conference on Optical Fiber Sensors, 2014, Santander, Spain
Abstract
Real-time observation of intracellular process of signal transduction is very useful for biomedical and pharmaceutical applications as well as for basic research work of cell biology. For feasible and reagentless observation of intracellular alterations in real time, we examined the use of a nonadiabatic tapered optical fiber (NATOF) biosensor for monitoring of intracellular signal transduction that was mainly translocation of protein kinase C via refractive index change in PC12 cells adhered on tapered fiber sensor without any indicator reagent. PC12 cells were stimulated with KCl . Our results suggest that complex intracellular reactions could be real-time monitored and characterized by NATOF biosensor.
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M. I. Zibaii, H. Latifi, A. Asadollahi, Z. Noraeipoor, L. Dargahi, "Real-time monitoring of intracellular signal transduction in PC12 cells by non-adiabatic tapered optical fiber biosensor", Proc. SPIE 9157, 23rd International Conference on Optical Fibre Sensors, 91574I (2 June 2014); doi: 10.1117/12.2058939; https://doi.org/10.1117/12.2058939
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