16 September 2014 The Pocketscope: a spatial light modulator based epi-fluorescence microscope for optogenetics
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Abstract
Microscopy incorporating spatial light modulators (SLMs) enables three dimensional (3D) excitation and monitoring of the activity of neuronal ensembles, enabling studies of neuronal circuit activity both in vitro and in vivo. In this paper we present a portable (22 cm x 42.5 cm x 30 cm), SLM-based epi-fluorescence upright microscope (“Pocketscope”) that enables 3D calcium imaging and photoactivation of neurons in brain slices. Here we describe the implementation of the instrument; quantify the volume over which neural activity can be excited; and demonstrate the use of the system for mapping neural circuits in brain slices.
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Anna Linnenberger, Darcy S. Peterka, Sean Quirin, Rafael Yuste, "The Pocketscope: a spatial light modulator based epi-fluorescence microscope for optogenetics", Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 91640Y (16 September 2014); doi: 10.1117/12.2064304; https://doi.org/10.1117/12.2064304
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