16 September 2014 Viscoelasticity measurements inside liposomes
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Microrheology, the study of the behavior of fluids on the microscopic scale, has been and continues to be one of the most important subjects that can be applied to characterize the behavior of biological fluids. It is extremely difficult to make rapid measurement of the viscoelastic properties of the interior of living cells. Liposomes are widely used as model system for studying different aspects of cell biology. We propose to develop a microrheometer, based on real-time control of optical tweezers, in order to investigate the viscoelastic properties of the fluid inside liposomes. This will give greater understanding of the viscoelastic properties of the fluids inside cells. In our experiment, the liposomes are prepared by different methods to find out both a better way to make GUVs and achieve efficient encapsulation of particle. By rotating the vaterite inside a liposome via spin angular momentum, the optical torque can be measured by measuring the change of polarization of the transmitted light, which allows the direct measurement of viscous drag torque since the optical torque is balanced by the viscous drag. We present an initial feasibility demonstration of trapping and manipulation of a microscopic vaterite inside the liposome. The applied method is simple and can be extended to sensing within the living cells.
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Shu Zhang, Shu Zhang, Lachlan Gibson, Lachlan Gibson, Daryl Preece, Daryl Preece, Timo A. Nieminen, Timo A. Nieminen, Halina Rubinsztein-Dunlop, Halina Rubinsztein-Dunlop, "Viscoelasticity measurements inside liposomes", Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 91642O (16 September 2014); doi: 10.1117/12.2060938; https://doi.org/10.1117/12.2060938

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