16 September 2014 Multiplexed spectroscopy with holographic optical tweezers
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We have developed a multiplexed holographic optical tweezers system with an imaging spectrometer to manipulate multiple optically trapped nanosensors and detect multiple fluorescence spectra. The system uses a spatial light modulator (SLM) to control the positions of infrared optical traps in the sample so that multiple nanosensors can be positioned into regions of interest. Spectra of multiple nanosensors are detected simultaneously with the application of an imaging spectrometer. Nanosensors are capable of detecting changes in their environment such as pH, ion concentration, temperature, and voltage by monitoring changes in the nanosensors' emitted fluorescence spectra. We use streptavidin labeled quantum dots bound to the surface of biotin labeled polystyrene microspheres to measure temperature changes by observing a corresponding shift in the wavelength of the spectral peak. The fluorescence is excited at 532 nm with a wide field source.
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Matthew A. Cibula, Matthew A. Cibula, David H. McIntyre, David H. McIntyre, "Multiplexed spectroscopy with holographic optical tweezers", Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 91642T (16 September 2014); doi: 10.1117/12.2061052; https://doi.org/10.1117/12.2061052


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