Anomalous behavior of a three-dimensional, optically trapped, super-paramagnetic particle

Martin Siler; Petr Jákl; Oto Brzobohatý; Jan Jezek; Pavel Zemánek

doi:10.1117/12.2062148

16 September 2014 Anomalous behavior of a three-dimensional, optically trapped, super-paramagnetic particle

Martin Siler, Petr Jákl, Oto Brzobohatý, Jan Jezek, Pavel Zemánek

Proceedings Volume 9164, Optical Trapping and Optical Micromanipulation XI; 916436 (2014) https://doi.org/10.1117/12.2062148
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

Super-paramagnetic particles are used extensively in diagnostics and other research applications for the purification of cells, biomolecules and assays. Here, we demonstrate full 3D optical manipulation of such sub-micrometer sized particle using optical tweezers. We report three types of anomalous behavior of such a trapped particle. Firstly, the analysis of particle motion recorded by the quadrant photodiode reveals spikes in the total detected intensity that are formed by a set of very fast oscillations. Secondly, the dependence of trap stiffness on the laser power deviates from the expected linear increase for higher trapping powers. And further, such a particle cannot be optically trapped above certain trapping power threshold. We speculate that these effects are caused by particle internal structure and by its heating.

Citation Download Citation

Martin Siler, Petr Jákl, Oto Brzobohatý, Jan Jezek, and Pavel Zemánek "Anomalous behavior of a three-dimensional, optically trapped, super-paramagnetic particle", Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 916436 (16 September 2014); https://doi.org/10.1117/12.2062148

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