28 August 2014 Using optical lattice for STED parallelization
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Abstract
Being a scanning microscopy, Stimulated Emission Depletion (STED) needs to be parallelized for fast wide-field imaging. Here, we achieve large parallelization of STED microscopy using well-designed Optical Lattice (OL) for depletion, together with a fast camera for detection. Depletion optical lattices with 100 intensity “zeros” are generated by four-beam interference. Scanning only a unit cell, as small as 290 nm by 290 nm, of the depletion OL is sufficient for STED imaging. The OL-STED microscopy acquires super-resolution images with 70 nm resolution and at the speed of 80 ms per image.
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Bin Yang, Bin Yang, Frédéric Przybilla, Frédéric Przybilla, Michael Mestre, Michael Mestre, Jean-Baptiste Trebbia, Jean-Baptiste Trebbia, Brahim Lounis, Brahim Lounis, "Using optical lattice for STED parallelization", Proc. SPIE 9169, Nanoimaging and Nanospectroscopy II, 91690E (28 August 2014); doi: 10.1117/12.2064820; https://doi.org/10.1117/12.2064820
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