Two-photon three-axis digital scanned light-sheet microscopy (2P3A-DSLM)

Weijian Zong; Jia Zhao; Xuanyang Chen; Yuan Lin; Huixia Ren; Yunfeng Zhang; Ming Fan; Zhuan Zhou; Heping Cheng; Yujie Sun; Liangyi Chen

doi:10.1117/12.2062690

5 September 2014 Two-photon three-axis digital scanned light-sheet microscopy (2P3A-DSLM)

Weijian Zong, Jia Zhao, Xuanyang Chen, Yuan Lin, Huixia Ren, Yunfeng Zhang, Ming Fan, Zhuan Zhou, Heping Cheng, Yujie Sun, Liangyi Chen

Author Affiliations +

Proceedings Volume 9198, Ultrafast Nonlinear Imaging and Spectroscopy II; 91980W (2014) https://doi.org/10.1117/12.2062690
Event: SPIE Optical Engineering + Applications, 2014, San Diego, California, United States

Abstract

In this presentation we report a new 3D scanned DSLM. The system combined 1) two-photon excitation, 2) scanning along the illumination axis (x-axis) using tunable acoustic gradient lens (TAG) to stretch the Rayleigh range [5], 3) scanning vertically to the illumination axis (y-axis) by one galvo mirror to create light sheet. 4) scanning along Z-axis to do fast 3D imaging by another galvo mirror. The image plane was kept aligned with the fast z-axis scanned light sheet plane by an electric tunable lens (ETL) as described in ref. 6. The light sheet can be tailored to any shape between 50×50 μm2 and more than 500×500 μm2 with constant thickness limited by diffraction and fast imaging rates limited by the detector. The tailorable illumination area allows multi-scale field of view (FOV), and is consequently capable of imaging cells, tissue and live animals in one setup.

Citation Download Citation

Weijian Zong, Jia Zhao, Xuanyang Chen, Yuan Lin, Huixia Ren, Yunfeng Zhang, Ming Fan, Zhuan Zhou, Heping Cheng, Yujie Sun, and Liangyi Chen "Two-photon three-axis digital scanned light-sheet microscopy (2P3A-DSLM)", Proc. SPIE 9198, Ultrafast Nonlinear Imaging and Spectroscopy II, 91980W (5 September 2014); https://doi.org/10.1117/12.2062690

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