11 September 2014 Grating interferometry-based phase microtomography of atherosclerotic human arteries
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Cardiovascular diseases are the number one cause of death and morbidity in the world. Understanding disease development in terms of lumen morphology and tissue composition of constricted arteries is essential to improve treatment and patient outcome. X-ray tomography provides non-destructive three-dimensional data with micrometer-resolution. However, a common problem is simultaneous visualization of soft and hard tissue-containing specimens, such as atherosclerotic human coronary arteries. Unlike absorption based techniques, where X-ray absorption strongly depends on atomic number and tissue density, phase contrast methods such as grating interferometry have significant advantages as the phase shift is only a linear function of the atomic number. We demonstrate that grating interferometry-based phase tomography is a powerful method to three-dimensionally visualize a variety of anatomical features in atherosclerotic human coronary arteries, including plaque, muscle, fat, and connective tissue. Three formalin-fixed, human coronary arteries were measured using advanced laboratory μCT. While this technique gives information about plaque morphology, it is impossible to extract the lumen morphology. Therefore, selected regions were measured using grating based phase tomography, sinograms were treated with a wavelet-Fourier filter to remove ring artifacts, and reconstructed data were processed to allow extraction of vessel lumen morphology. Phase tomography data in combination with conventional laboratory μCT data of the same specimen shows potential, through use of a joint histogram, to identify more tissue types than either technique alone. Such phase tomography data was also rigidly registered to subsequently decalcified arteries that were histologically sectioned, although the quality of registration was insufficient for joint histogram analysis.
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Marzia Buscema, Marzia Buscema, Margaret N. Holme, Margaret N. Holme, Hans Deyhle, Hans Deyhle, Georg Schulz, Georg Schulz, Rüdiger Schmitz, Rüdiger Schmitz, Peter Thalmann, Peter Thalmann, Simone E. Hieber, Simone E. Hieber, Natalia Chicherova, Natalia Chicherova, Philippe C. Cattin, Philippe C. Cattin, Felix Beckmann, Felix Beckmann, Julia Herzen, Julia Herzen, Timm Weitkamp, Timm Weitkamp, Till Saxer, Till Saxer, Bert Müller, Bert Müller, } "Grating interferometry-based phase microtomography of atherosclerotic human arteries", Proc. SPIE 9212, Developments in X-Ray Tomography IX, 921203 (11 September 2014); doi: 10.1117/12.2060443; https://doi.org/10.1117/12.2060443

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