11 September 2014 X-ray phase contrast tomography from whole organ down to single cells
Author Affiliations +
Abstract
We use propagation based hard x-ray phase contrast tomography to explore the three dimensional structure of neuronal tissues from the organ down to sub-cellular level, based on combinations of synchrotron radiation and laboratory sources. To this end a laboratory based microfocus tomography setup has been built in which the geometry was optimized for phase contrast imaging and tomography. By utilizing phase retrieval algorithms, quantitative reconstructions can be obtained that enable automatic renderings without edge artifacts. A high brightness liquid metal microfocus x-ray source in combination with a high resolution detector yielding a resolution down to 1.5 μm. To extend the method to nanoscale resolution we use a divergent x-ray waveguide beam geometry at the synchrotron. Thus, the magnification can be easily tuned by placing the sample at different defocus distances. Due to the small Fresnel numbers in this geometry the measured images are of holographic nature which poses a challenge in phase retrieval.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Martin Krenkel, Martin Krenkel, Mareike Töpperwien, Mareike Töpperwien, Matthias Bartels, Matthias Bartels, Paul Lingor, Paul Lingor, Detlev Schild, Detlev Schild, Tim Salditt, Tim Salditt, } "X-ray phase contrast tomography from whole organ down to single cells", Proc. SPIE 9212, Developments in X-Ray Tomography IX, 92120R (11 September 2014); doi: 10.1117/12.2060390; https://doi.org/10.1117/12.2060390
PROCEEDINGS
13 PAGES


SHARE
Back to Top