17 September 2014 Determination of nitric oxide mediating intracellular Ca2+ release on neurons based on confocal microscopy imaging
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Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 92301F (2014) https://doi.org/10.1117/12.2068890
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China
Abstract
The gas NO is a ubiquitous intercellular messenger that modulates a wide range of physiological and pathophysiological functions. But few studies were made to study the role of NO in the Ca2+ release in dorsal root ganglion (DRG) neurons by confocal microscopy. Thus the objective of this study was to assess if NO has a role in Ca2+ signaling in DRG neurons using confocal microscopy combined with special fluorescence probe Fluo-3/AM. A 100 μM concentration of the NO donors (Sodium Nitroprusside, Dihydrate, SNP) and NO synthase inhibitor (NG-Monomethyl-L-arginine, Monoacetate salt, L-NMMA) was used in the study. Results showed that the fluorescence intensity increased rapidly after injecting SNP, which indicated that SNP could enhance intracellular Ca2+ release. And the fluorescence intensity shrank gradually with time and kept at a low level for quite a long period after loading with L-NMMA which indicated that L-NMMA could block intracellular Ca2+ release. All these results demonstrated that NO was involved in the regulation of intracellular Ca2+ release in the DRG neurons.
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Liqin Zheng, Yuhua Wang, Yipeng He, Yixiu Zeng, Yanding Zhang, Shusen Xie, "Determination of nitric oxide mediating intracellular Ca2+ release on neurons based on confocal microscopy imaging", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301F (17 September 2014); doi: 10.1117/12.2068890; https://doi.org/10.1117/12.2068890
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