17 September 2014 Serum albumin analysis for type II diabetes detection using surface-enhanced Raman spectroscopy
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Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 92301Z (2014) https://doi.org/10.1117/12.2068851
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China
Abstract
Surface-enhanced Raman scattering (SERS) spectroscopy combined with membrane electrophoresis (ME) was firstly employed to detect albumin variation in type II diabetic development. Albumin was first purified from human serum by ME and then mixed with silver nanoparticles to perform SERS spectral analysis. SERS spectra were obtained from blood albumin samples of 20 diabetic patients and 19 healthy volunteers. Subtle but discernible changes in the acquired mean spectra of the two groups were observed. Tentative assignment of albumin SERS bands indicated specific structural changes of albumin molecule with diabetic development. Meanwhile, PCA-LDA diagnostic algorithms were employed to classify the two kinds of albumin SERS spectra, yielding the diagnostic sensitivity of 90% and specificity of 94.7%. The results from this exploratory study demonstrated that the EM-SERS method in combination with multivariate statistical analysis has great potential for the label-free detection of albumin variation for improving type II diabetes screening.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jinyong Lin, Jinyong Lin, Gang Cao, Gang Cao, Juqiang Lin, Juqiang Lin, Nenrong Liu, Nenrong Liu, Fadian Liao, Fadian Liao, Qiuyong Ruan, Qiuyong Ruan, Shanshan Wu, Shanshan Wu, Zufang Huang, Zufang Huang, Ling Li, Ling Li, Rong Chen, Rong Chen, } "Serum albumin analysis for type II diabetes detection using surface-enhanced Raman spectroscopy", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301Z (17 September 2014); doi: 10.1117/12.2068851; https://doi.org/10.1117/12.2068851
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