12 August 1988 Derivative Analysis Of Spectral Absorption By Phytoplankton Pigments
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Concurrent measurements of the spectral absorption coefficient and photosynthetic pigmentation of natural particulates were performed to determine the principal pigments responsible for, the absorption of spectral irradiance in seawater. The spectral absorption coefficient, Ap(λ), was analyzed by taking the second and fourth derivatives with respect to wavelength. The wavelength and magnitude of these derivative values provide useful information regarding the identification and quantification of phytoplankton pigments responsible for a given spectral signature. Linear relationships were examined and established between derivative values at selected wavelengths and concentrations of the major tetrapyrrole pigments, specifically chlorophylls a, b, and c. The method described here provides *a rapid means of obtaining estimates of photosynthetic pigment concentrations in natural samples where absorption can be strongly influenced by detrital matter.
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Robert R Bidigare, Robert R Bidigare, John H Morrow, John H Morrow, Dale A Kiefer, Dale A Kiefer, } "Derivative Analysis Of Spectral Absorption By Phytoplankton Pigments", Proc. SPIE 0925, Ocean Optics IX, (12 August 1988); doi: 10.1117/12.945713; https://doi.org/10.1117/12.945713

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