Focusing criterion in DHM image reconstruction

M. Mihailescu; N. Mihale; R. C. Popescu; A. Acasandrei; I. A. Paun; M. Dinescu; E. Scarlat

doi:10.1117/12.2070450

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20 February 2015 Focusing criterion in DHM image reconstruction

M. Mihailescu; N. Mihale; R. C. Popescu; A. Acasandrei; I. A. Paun; M. Dinescu; E. Scarlat

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Proceedings Volume 9258, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies VII; 92580U (2015) https://doi.org/10.1117/12.2070450
Event: Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies 2014, 2014, Constanta, Romania

Abstract

This study is presenting the theoretical approach and the practical results of a precise activity involved in the hologram reconstruction in order to find the optimally focused image of MG63 osteoblast-like cells cultivated on polymeric flat substrates. The morphology and dynamic of the cell is investigated by digital holographic microscopy (DHM) technique. The reconstruction is digitally performed using an algorithm based on the scalar theory of diffraction in the Fresnel approximation. The quality of the 3D images of the cells is crucially depending on the focusing capability of the reconstruction chain to fit the parameters of the optical recorder, particularly the focusing value. Our proposal to find the focused image is based on the images decomposition on gray levels and their histogram analysis. More precisely the focusing criterion is based on the evaluation of the form of this distribution.

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M. Mihailescu, N. Mihale, R. C. Popescu, A. Acasandrei, I. A. Paun, M. Dinescu, and E. Scarlat "Focusing criterion in DHM image reconstruction", Proc. SPIE 9258, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies VII, 92580U (20 February 2015); https://doi.org/10.1117/12.2070450

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