Wide field-of-view microscopy using compressive sensing

Jie Wang; Jigang Wu

doi:10.1117/12.2071601

18 November 2014 Wide field-of-view microscopy using compressive sensing

Jie Wang, Jigang Wu

Author Affiliations +

Proceedings Volume 9268, Optics in Health Care and Biomedical Optics VI; 926811 (2014) https://doi.org/10.1117/12.2071601
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

Focus-grid-based wide field-of-view microscope has been developed to break the trade-off of resolution and field of view in conventional microscopy. In the wide field-of-view microscopy, the whole sample area has to be scanned with at least Nyquist-frequency sampling for image reconstruction. We propose a novel scanning mechanism using compressive sensing (CS), where the scanning of focal spots covers only a portion of the sample. Our preliminary studies show that 75% of scanning area is enough to reconstruct a decent image. Thus we can reduce the acquisition data which allow for simpler scanning mechanism and data acquisition.

Citation Download Citation

Jie Wang, Jie Wang, Jigang Wu, Jigang Wu, "Wide field-of-view microscopy using compressive sensing", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 926811 (18 November 2014); doi: 10.1117/12.2071601; https://doi.org/10.1117/12.2071601

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