Homing peptide guiding optical molecular imaging for the diagnosis of bladder cancer

Xiao-feng Yang; Jian-zhi Pang; Jie-hao Liu; Yang Zhao; Xing-you Jia; Jun Li; Reng-xin Liu; Wei Wang; Zhen-wei Fan; Zi-qiang Zhang; San-hua Yan; Jun-qian Luo; Xiao-lei Zhang

doi:10.1117/12.2071227

20 November 2014 Homing peptide guiding optical molecular imaging for the diagnosis of bladder cancer

Xiao-feng Yang, Jian-zhi Pang, Jie-hao Liu, Yang Zhao, Xing-you Jia, Jun Li, Reng-xin Liu, Wei Wang, Zhen-wei Fan, Zi-qiang Zhang, San-hua Yan, Jun-qian Luo, Xiao-lei Zhang

Author Affiliations +

Proceedings Volume 9268, Optics in Health Care and Biomedical Optics VI; 92681P (2014) https://doi.org/10.1117/12.2071227
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

Background: The limitations of primary transurethral resection of bladder tumor (TURBt) have led the residual tumors rates as high as 75%. The intraoperative fluorescence imaging offers a great potential for improving TURBt have been confirmed. So we aim to distinguish the residual tumors and normal mucosa using fluorescence molecular imaging formed by conjugated molecule of the CSNRDARRC bladder cancer homing peptide with fluorescent dye. The conjugated molecule was abbreviated FIuo-ACP. In our study, we will research the image features of FIuo-ACP probe targeted bladder cancer for fluorescence molecular imaging diagnosis for bladder cancer in vivo and ex vivo. Methods: After the FIuo-ACP probe was synthetized, the binding sites, factors affecting binding rates, the specificity and the targeting of Fluo-ACP labeled with bladder cancer cells were studied respectively by laser scanning confocal microscope (LSCM), immunofluorescence and multispectral fluorescence ex vivo optical molecular imaging system. Results: The binding sites were located in nucleus and the binding rates were correlated linearly with the dose of probe and the grade of pathology. Moreover, the probe has a binding specificity with bladder cancer in vivo and ex vivo. Tumor cells being labeled by the Fluo-ACP, bright green spots were observed under LSCM. The tissue samples and tumor cells can be labeled and identified by fluorescence microscope. Optical molecular imaging of xenograft tumor tissues was exhibited as fluorescent spots under EMCCD. Conclusion: The CSNRDARRC peptides might be a useful bladder cancer targeting vector. The FIuo-ACP molecular probe was suitable for fluorescence molecular imaging diagnosis for bladder cancer in vivo and ex vivo.

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Xiao-feng Yang, Jian-zhi Pang, Jie-hao Liu, Yang Zhao, Xing-you Jia, Jun Li, Reng-xin Liu, Wei Wang, Zhen-wei Fan, Zi-qiang Zhang, San-hua Yan, Jun-qian Luo, and Xiao-lei Zhang "Homing peptide guiding optical molecular imaging for the diagnosis of bladder cancer", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 92681P (20 November 2014); https://doi.org/10.1117/12.2071227

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KEYWORDS

Bladder cancer

Luminescence

Tumors

Molecular imaging

Tissues

Molecules

Bladder

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