20 November 2014 Homing peptide guiding optical molecular imaging for the diagnosis of bladder cancer
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Abstract
Background: The limitations of primary transurethral resection of bladder tumor (TURBt) have led the residual tumors rates as high as 75%. The intraoperative fluorescence imaging offers a great potential for improving TURBt have been confirmed. So we aim to distinguish the residual tumors and normal mucosa using fluorescence molecular imaging formed by conjugated molecule of the CSNRDARRC bladder cancer homing peptide with fluorescent dye. The conjugated molecule was abbreviated FIuo-ACP. In our study, we will research the image features of FIuo-ACP probe targeted bladder cancer for fluorescence molecular imaging diagnosis for bladder cancer in vivo and ex vivo. Methods: After the FIuo-ACP probe was synthetized, the binding sites, factors affecting binding rates, the specificity and the targeting of Fluo-ACP labeled with bladder cancer cells were studied respectively by laser scanning confocal microscope (LSCM), immunofluorescence and multispectral fluorescence ex vivo optical molecular imaging system. Results: The binding sites were located in nucleus and the binding rates were correlated linearly with the dose of probe and the grade of pathology. Moreover, the probe has a binding specificity with bladder cancer in vivo and ex vivo. Tumor cells being labeled by the Fluo-ACP, bright green spots were observed under LSCM. The tissue samples and tumor cells can be labeled and identified by fluorescence microscope. Optical molecular imaging of xenograft tumor tissues was exhibited as fluorescent spots under EMCCD. Conclusion: The CSNRDARRC peptides might be a useful bladder cancer targeting vector. The FIuo-ACP molecular probe was suitable for fluorescence molecular imaging diagnosis for bladder cancer in vivo and ex vivo.
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Xiao-feng Yang, Xiao-feng Yang, Jian-zhi Pang, Jian-zhi Pang, Jie-hao Liu, Jie-hao Liu, Yang Zhao, Yang Zhao, Xing-you Jia, Xing-you Jia, Jun Li, Jun Li, Reng-xin Liu, Reng-xin Liu, Wei Wang, Wei Wang, Zhen-wei Fan, Zhen-wei Fan, Zi-qiang Zhang, Zi-qiang Zhang, San-hua Yan, San-hua Yan, Jun-qian Luo, Jun-qian Luo, Xiao-lei Zhang, Xiao-lei Zhang, } "Homing peptide guiding optical molecular imaging for the diagnosis of bladder cancer", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 92681P (20 November 2014); doi: 10.1117/12.2071227; https://doi.org/10.1117/12.2071227
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