20 November 2014 In-vivo monitoring rat skin wound healing using nonlinear optical microscopy
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Nonlinear optical microscopy (NLOM) was employed for imaging and evaluating the wound healing process on rat skin in vivo. From the high-resolution nonlinear optical images, the morphology and distribution of specific biological markers in cutaneous wound healing such as fibrin clot, collagens, blood capillaries, and hairs were clearly observed at 1, 5 and 14 days post injury. We found that the disordered collagen in the fibrin clot at day 1 was replaced by regenerative collagen at day 5. By day 14, the thick collagen with well-network appeared at the original margin of the wound. These findings suggested that NLOM is ideal for noninvasively monitoring the progress of wound healing in vivo.
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Jing Chen, Jing Chen, Chungen Guo, Chungen Guo, Fan Zhang, Fan Zhang, Yahao Xu, Yahao Xu, Xiaoqin Zhu, Xiaoqin Zhu, Shuyuan Xiong, Shuyuan Xiong, Jianxin Chen, Jianxin Chen, "In-vivo monitoring rat skin wound healing using nonlinear optical microscopy", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 926820 (20 November 2014); doi: 10.1117/12.2071547; https://doi.org/10.1117/12.2071547

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