Multiphoton microscopic imaging of fibrotic focus in invasive ductal carcinoma of the breast

Sijia Chen; Yuting Nie; Yuane Lian; Yan Wu; Fangmeng Fu; Chuan Wang; Shuangmu Zhuo; Jianxin Chen

doi:10.1117/12.2071626

20 November 2014 Multiphoton microscopic imaging of fibrotic focus in invasive ductal carcinoma of the breast

Sijia Chen, Yuting Nie, Yuane Lian, Yan Wu, Fangmeng Fu, Chuan Wang, Shuangmu Zhuo, Jianxin Chen

Proceedings Volume 9268, Optics in Health Care and Biomedical Optics VI; 926823 (2014) https://doi.org/10.1117/12.2071626
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

During the proliferation of breast cancer, the desmoplastic can evoke a fibrosis response by invading healthy tissue. Fibrotic focus (FF) in invasive ductal carcinoma (IDC) of the breast had been reported to be associated with significantly poorer survival rate than IDC without FF. As an important prognosis indicator, it’s difficult to obtain the exact fibrotic information from traditional detection method such as mammography. Multiphoton imaging based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) has been recently employed for microscopic examination of unstained tissue. In this study, multiphoton microscopy (MPM) was used to image the fibrotic focus in invasive ductal carcinoma tissue. The morphology and distribution of collagen in fibrotic focus can be demonstrated by the SHG signal. Variation of collagen between IDC with and without FF will be examined and further characterized, which may be greatly related to the metastasis of breast cancer. Our result suggested that the MPM can be efficient in identifying and locating the fibrotic focus in IDC. Combining with the pathology analysis and other detecting methods, MPM owns potential in becoming an advanced histological tool for detecting the fibrotic focus in IDC and collecting prognosis information, which may guide the subsequent surgery option and therapy procedure for patients.

Citation Download Citation

Sijia Chen, Yuting Nie, Yuane Lian, Yan Wu, Fangmeng Fu, Chuan Wang, Shuangmu Zhuo, and Jianxin Chen "Multiphoton microscopic imaging of fibrotic focus in invasive ductal carcinoma of the breast", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 926823 (20 November 2014); https://doi.org/10.1117/12.2071626

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KEYWORDS

Collagen

Tumors

Second-harmonic generation

Breast

Tissues

Breast cancer

Imaging systems

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