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20 November 2014 Multiphoton microscopic imaging of fibrotic focus in invasive ductal carcinoma of the breast
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During the proliferation of breast cancer, the desmoplastic can evoke a fibrosis response by invading healthy tissue. Fibrotic focus (FF) in invasive ductal carcinoma (IDC) of the breast had been reported to be associated with significantly poorer survival rate than IDC without FF. As an important prognosis indicator, it’s difficult to obtain the exact fibrotic information from traditional detection method such as mammography. Multiphoton imaging based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) has been recently employed for microscopic examination of unstained tissue. In this study, multiphoton microscopy (MPM) was used to image the fibrotic focus in invasive ductal carcinoma tissue. The morphology and distribution of collagen in fibrotic focus can be demonstrated by the SHG signal. Variation of collagen between IDC with and without FF will be examined and further characterized, which may be greatly related to the metastasis of breast cancer. Our result suggested that the MPM can be efficient in identifying and locating the fibrotic focus in IDC. Combining with the pathology analysis and other detecting methods, MPM owns potential in becoming an advanced histological tool for detecting the fibrotic focus in IDC and collecting prognosis information, which may guide the subsequent surgery option and therapy procedure for patients.
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Sijia Chen, Yuting Nie, Yuane Lian, Yan Wu, Fangmeng Fu, Chuan Wang, Shuangmu Zhuo, and Jianxin Chen "Multiphoton microscopic imaging of fibrotic focus in invasive ductal carcinoma of the breast", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 926823 (20 November 2014);

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