11 November 2014 Lensless microscope based on iterative in-line holographic reconstruction
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Abstract
We propose a lensless microscopic imaging technique based on iteration algorithm with known constraint for image reconstruction in digital in-line holography. In our method, we introduce a constraint on the sample plane as known part in the lensless microscopy for iteration algorithm in order to eliminate the twin-image effect of holography and thus lead to better performance on microscopic imaging. We evaluate our method by numerical simulation and built a prototype in-line holographic imaging system and demonstrated its capability by preliminary experiments. In our proposed setup, a carefully designed photomask used to hold the sample is under illumination of a coherent light source. The in-line hologram is then recorded by a CMOS sensor. In the reconstruction, the known information of the illumination beam and the photomask is used as constraints in the iteration process. The improvement of image quality because of suppression of twin-images can be clearly seen by comparing the images obtained by direct holographic reconstruction and our iterative method.
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Jigang Wu, Jigang Wu, } "Lensless microscope based on iterative in-line holographic reconstruction", Proc. SPIE 9271, Holography, Diffractive Optics, and Applications VI, 927114 (11 November 2014); doi: 10.1117/12.2071890; https://doi.org/10.1117/12.2071890
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