5 November 2014 Imaging microscopy by phase-contrast engine: retardation-modulated differential interference contrast microscope
Author Affiliations +
Abstract
In the field of biology and medicine, observation object of the microscope has been changing from the thin specimen to the thick living tissue. Furthermore, observation of the internal structure of a living tissue is also desired by low invasion. However, the real structure of a phase object with three-dimensional distribution such as a living tissue is difficult to observe, because of the influence of the phase distribution before and behind of observation position. We enabled observation of the internal structure of living tissue without stain, by adding a new function to reduce the influence of phase distribution to our Retardation-Modulated differential interference contrast (RM-DIC) microscope system.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Hiroshi Ishiwata, Hiroshi Ishiwata, Masahide Itoh, Masahide Itoh, } "Imaging microscopy by phase-contrast engine: retardation-modulated differential interference contrast microscope", Proc. SPIE 9272, Optical Design and Testing VI, 92720A (5 November 2014); doi: 10.1117/12.2074378; https://doi.org/10.1117/12.2074378
PROCEEDINGS
5 PAGES


SHARE
Back to Top