Translator Disclaimer
5 November 2014 Phase-contrast scanning optical microscopy for biological tissues
Author Affiliations +
Phase-contrast imaging of living tissues is important for investigating biological structures and functions. The phasecontrast scanning optical microscope using annular illumination and image processing has been developed as a new tool for visualizing phase objects. This microscope has excellent features, such as separate phase and amplitude imaging, reducing multiscattering effects and spherical aberration-free imaging. Using a figure-8-shaped spatial-frequency filter with sign correction in order to extract linear phase-contrast components, we observed the phase-contrast structures of biological tissue samples and evaluated the microscope’s imaging features.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Masaki Hisaka "Phase-contrast scanning optical microscopy for biological tissues", Proc. SPIE 9272, Optical Design and Testing VI, 92720B (5 November 2014);

Back to Top