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29 October 2014Light field creating and imaging with different order intensity derivatives
Microscopic image restoration and reconstruction is a challenging topic in the image processing and computer vision,
which can be widely applied to life science, biology and medicine etc. A microscopic light field creating and three
dimensional (3D) reconstruction method is proposed for transparent or partially transparent microscopic samples, which
is based on the Taylor expansion theorem and polynomial fitting. Firstly the image stack of the specimen is divided into
several groups in an overlapping or non-overlapping way along the optical axis, and the first image of every group is
regarded as reference image. Then different order intensity derivatives are calculated using all the images of every group
and polynomial fitting method based on the assumption that the structure of the specimen contained by the image stack
in a small range along the optical axis are possessed of smooth and linear property. Subsequently, new images located
any position from which to reference image the distance is Δz along the optical axis can be generated by means of
Taylor expansion theorem and the calculated different order intensity derivatives. Finally, the microscopic specimen can
be reconstructed in 3D form using deconvolution technology and all the images including both the observed images and
the generated images. The experimental results show the effectiveness and feasibility of our method.
Yu Wang andHuan Jiang
"Light field creating and imaging with different order intensity derivatives", Proc. SPIE 9273, Optoelectronic Imaging and Multimedia Technology III, 92730Z (29 October 2014); https://doi.org/10.1117/12.2071428
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Yu Wang, Huan Jiang, "Light field creating and imaging with different order intensity derivatives," Proc. SPIE 9273, Optoelectronic Imaging and Multimedia Technology III, 92730Z (29 October 2014); https://doi.org/10.1117/12.2071428